Aakanksha Singh was born on 30 July 1990 in Jaipur, Rajasthan. Her mother is a theatre artist. She completed her schooling from Ryan International School and is a physiotherapist by education. She started her career with theatre and made her acting debut in 2012 with the television show Na Bole Tum Na Maine Kuch Kaha (2012–2014). She portrayed Megha Vyas Bhatnagar, a widow with two children opposite Kunal Karan Kapoor. She won Indian Telly Award for Fresh New Face - Female for her performance. The show ended in 2013, with two seasons.
She next appeared in the finite series, Gulmohar Grand in 2015. The show based on the hotel industry saw her portraying Anahita "Annie" Mehta Fernandez. Aakanksha has also been part of Box Cricket League for the team Ahmedabad Express in 2016. In 2017, she played a lawyer in an episode of Aye Zindagi.
Aakanksha made her film debut with the Hindi film Badrinath Ki Dulhania in 2017. In the same year she made her Telugu debut with Malli Raava opposite Sumanth. It received mixed to positive reviews and she got nominated with SIIMA Award for Best Female Debut – Telugu for her performance.
In 2018, she appeared in her second Telugu film Devadas opposite Nagarjuna. It received positive reviews and was a commercial success. The same year, she appeared in two non- Telugu short films Methi Ke Laddoo and Qaid.
Aakanksha marked her Kannada film debut with the 2019 film Pailwaan opposite Sudeep. It was released in 5 languages and was an average hit at the box office. In 2021, she made her web debut with the Telugu web series Parampara that premiered on Disney+ Hotstar.
Aakanksha made her Tamil debut in 2022 with the Tamil-Telugu bilingual film, Clap opposite Aadhi Pinisetty that released on SonyLIV platform. She then appeared in the Tamil film Veerapandiyapuram which received negative reviews.
She next played Ajay Devgn's wife in the Hindi film Runway 34, which marks her second Hindi film. It received mixed to positive reviews. She portrayed a cop in her first Hindi web series Escaype Live on Disney+ Hotstar. She has signed for another Hindi web series Rangbaaz: Darr Ki Rajneeti opposite Viineet Kumar.
She has also appeared in the relently released Telugu web series "Meet Cute", an anthology produced by Nani, in the "In L(aw)ove" segment.
Aakanksha ranked 12th in Times of India's Bangalore Times 30 Most Desirable Women of 2019. She married her longtime boyfriend Kunal Sain, a marketing professional, in 2014 in Rajasthan. Aakanksha is a prominent celebrity endorser for brands and products.